3 matrix assisted laser desorption ionization time of flight mass spectrometry
Báo cáo khoa học: Reduction of S-nitrosoglutathione by human alcohol dehydrogenase 3 is an irreversible reaction as analysed by electrospray mass spectrometry pptx
... Electrospray mass and tandem mass spectra were recorded on an AutoSpec-OATOFFPD high-resolution magnetic sector-orthogonal acceleration time- of- flight (OATOF) tandem mass spectrometer (Micromass) Both ... activity of the human form of ADH3 for this compound Recombinant expression and purification of wild-type and mutated forms of the enzyme enabled kinetic characterization and investigation of substrate–enzyme ... determine the structure of the product generated by ADH3 catalysed reduction of GSNO, nano-electrospray mass spectra of the reaction mixture with and without the addition of enzyme were recorded...
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Ionization Methods in Organic Mass Spectrometry pot
... choice of ionization method depends on the nature of the sample and the type of information required from the analysis So-called 'soft ionization' methods such as field desorption and electrospray ionization ... analyzed by mass spectrometry Although ionization and desorption/ desolvation are usually separate processes, the term "ionization method" is commonly used to refer to both ionization and desorption ... to ESI Mass range Low-moderate Typically less than 2000 Da Laser Desorption Laser desorption methods use a pulsed laser to desorb species from a target surface Therefore, one must use a mass analyzer...
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báo cáo khoa học: " Analysis of expressed sequence tags from a single wheat cultivar facilitates interpretation of tandem mass spectrometry data and discrimination of gamma gliadin proteins that may play different functional roles in flour" ppt
... electrospray ionization; EST: expressed sequence tag; HMW: high molecular weight; LMW: low molecular weight; MALDI-TOF: matrix -assisted laser desorption/ ionization time of flight mass spectrometry; ... D: Characterization of B- and C-type low molecular weight glutenin subunits by electrospray ionization mass spectrometry and matrix -assisted laser desorption/ ionization mass spectrometry Proteomics ... effects of mineral nutrients and high temperature Eur J Agron 2006, 25:96-107 Ferranti P, Mamone G, Picariello G, Addeo F: Mass spectrometry analysis of gliadins in celiac disease J Mass Spectrom...
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planar laser imaging and modeling of matrix assisted pulsed laser
... to characterize bubble growth versus time for three different ink-film thicknesses of 12, 25, and 50 m and for two laser- beam diameters of 30 and 60 m The laser Downloaded 19 Jan 2007 to 128.46.184.20 ... droplet of ink at one end of a soda-lime glass slide between two metal shims A glass rod was then pulled across the two shims, creating a flat layer of ink at nearly the same thickness as that of the ... parameters, the bubble height as a function of time can be determined from numerical solution of Eq ͑4͒ Such solutions were obtained using an initial value of y equal to the initial vapor-pocket thickness...
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Tài liệu Real-time, on-line monitoring of organic chemical reactions using extractive electrospray ionization tandem mass spectrometry pdf
... which is carried out on a quadrupole time- of- flight (Q-TOF) mass spectrometer The relatively simple setup allows this method to be implemented on any type of MS instrument equipped with an ESI/APCI ... cone voltage was 40 V The Q-TOF mass spectrometer (QTOF UltimaTM, Micromass/Waters, Manchester, UK) was running in positive ion detection mode, Figure Schematic view of the EESI setup Copyright ... inlet of the mass spectrometer at a 408 angle from the axis of the sampling cone (shown in Fig 1) By introducing an intermittent, or if necessary continuous, N2 gas flow (50 L/h) through one neck of...
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Báo cáo y học: " Vacuum-assisted closure in the treatment of early hip joint infection"
... the transport of cellular and humoral components of the immune system to the infected area is facilitated, which leads to a significant reduction of the bacterial count The efficacy of the V.A.C ... inspections of the sponges can be compensated by daily controlling of the canister fluid and monitoring of the inflammation parameters Our clinical outcome with a success rate in 26 out of 28 cases ... arguable Hereby, the creation of a therapy concept is essential with respect to the treat- 246 ment strategies of septic surgery and the causes of the infection Conflict of Interest The authors have...
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Effects of time of heatsetting on the tensile properties of ingeo™ poly (lactic acid) (PLA) fabric
... Figure Effect of time of heatsetting on the linearity of load extension in weft direction of knitted Ingeo™ Poly (lactic acid) Figure Mean effect of time of heatsetting on the linearity of load extension ... Increasing time of heatsetting had effect on tensile properties of PLA at varying times of 30s to 90s at temperature of 130°C Optimum effect on tensile properties occurred at times of 30 – 45s ... 3.21 3.03 2.59 Figure Effect of time of heatsetting on tensile energy WT g.cm/cm2 in warp direction of knitted Ingeo Poly (lactic acid) fabric Figure 10 Effect of time of heatsetting on tensile...
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IELTS Part 2 and Part 3 Topics and Questions -The Cultural Impact of Overseas Travel
... or time of the year You should say: what season (or time of the year) it is what the weather is like at this time what you usually at this time and explain why you enjoy that season or time of ... these works of art What you think of this? • Sometimes works of art are sold for extremely high prices What you think of that? • How are decisions made about the (financial) value of works of art? ... example of a happy time when you took a photograph? • Do you think it's important for a person to have photographs of his or her life through the years? • What is the value of keeping photographs of...
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In the time of AIDS
... 2007 16:41 In the time of AIDS 15 In the time of AIDS 289 P1: RNK 0521864381 c13 CUNY780B-African 290 978 521 68297 May 15, 2007 16:41 africans: the history of a continent of the forty-seven ... paragon of financial prudence, was massively overlent to African governments Yet there were signs of recovery Whereas sub-Saharan Africa’s annual growth rate of GDP had fallen from its high level of ... leaders of the Movement for the Restoration of the Ten Commandments of God told their followers before P1: RNK 0521864381 c13 CUNY780B-African 978 521 68297 May 15, 2007 In the time of AIDS 16:41...
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Tài liệu Lab 3.1.1 Safe Handling and Use of a Multimeter docx
... Put the tip of the red, positive lead on the positive side of a battery Put the tip of the black, negative, lead on the other end of a battery a Is any number showing up on the multimeter? _If ... the red probe should go in the + (plus) jack Step Turn on the multimeter Click or turn to the on button a What is the model of multimeter? b What action must ... many different switch positions does the multimeter have? b What are they? Step Switch or turn the multimeter to the voltage measurement a What...
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Tài liệu Lab 3.1.1 Safe Handling and Use of a Multimeter doc
... Put the tip of the red, positive lead on the positive side of a battery Put the tip of the black, negative, lead on the other end of a battery a Is any number showing up on the multimeter? _If ... the red probe should go in the + (plus) jack Step Turn on the multimeter Click or turn to the on button a What is the model of multimeter? b What action must ... many different switch positions does the multimeter have? b What are they? Step Switch or turn the multimeter to the voltage measurement a What...
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Tài liệu Báo cáo khoa học: Comparison of membrane fraction proteomic profiles of normal and cancerous human colorectal tissues with gel-assisted digestion and iTRAQ labeling mass spectrometry pptx
... roles in the pathogenesis of CRC In general, factors that contribute to the pathogenesis of CRC include the accumulation of mutations and the deregulation of gene expression Of particular interest ... quantitation, we used multi-q [41] software to analyze the iTRAQ data Raw data files from the Waters Q-Tof premier mass spectrometer were converted into files of mzXML format using masswolf (Institute for ... partitioning of the expression profiles of identified proteins, the results from cluster groups and confirmed 73.8% (31 ⁄ 42) of the previously selected differentially expressed proteins (more than twofold...
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Tài liệu Báo cáo khoa học: Involvement of two positively charged residues of Chlamydomonas reinhardtii glyceraldehyde-3-phosphate dehydrogenase in the assembly process of a bi-enzyme complex involved in CO2 assimilation doc
... 4, lg of GAPDH ($ 0.08 nmol) and 0.08 lg of CP12 ($ 0.08 nmol) were mixed and lg of the mixture was analyzed In lane 2, 38 lg of the K128A mutant and lg of CP12 were mixed and about 10 lg of the ... of GAPDH, one dimer of PRK and one molecule of CP12) essential to the formation of the native complex by dimerization of this unit [29] Second, we have previously shown that the association of ... least twofold), even though it was not possible to have an accurate estimation of its value due to limitations of the spectrophotometer (standard errors of 20%) The catalytic rate constants of these...
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Tài liệu Disaster relief emergency fund (DREF) ZiThe Time of Our Lives: Life Span Development of Timing and Event Trackingmbabwe: Floods pdf
... explanation of our findings on the basis of an entrainment theory of life span development of timing and event tracking Figure provides a conceptual summary of our findings in terms of the two ... outlines a progression of expanding periodicities over time (i.e., a lifetime), which is lawful but not uniform Traditional views of age-related slowing The present findings offer a new perspective ... are meaningfully recast in light of the two entrainment hypotheses offered here if we distinguish between types of response time measures and different kinds of tasks Conclusion This research...
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Tài liệu Báo cáo khoa học: Crystal structure of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase complexed with an analogue of 1,3-bisphospho-D-glyceric acid Selective inhibition by structure-based design docx
... that improved activity of this compound may be a result of hydrogen bonding between the hydroxyl of Thr167 of the protein and the amino group of compound No other inhibitor offered such an interaction ... in the range of the Km values for 1,3-BPGA and even up to three times lower for compound Selection of T brucei gGAPDH residues to be mutated and measurement of kinetic parameters of the mutated ... conformation of the enzyme in the absence of substrate and cofactor Molecular modelling To elucidate the different behaviour of these inhibitors on the two trypanosomatid gGAPDHs, modelling studies of...
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Báo cáo khoa học: Modified PCR methods for 3¢ end amplification from serial analysis of gene expression (SAGE) tags doc
... volume of 28.6 lL Precipitation mixture I: lL of glycogen (20 mgÆmL)1; Roche, Indianapolis, IN), 15 lL of 7.5 m NH4Ac, and 84 lL of 100% ethanol Precipitation mixture II: 22 lL of H2O, 15 lL of m ... tag-binding site (B) The result of GLGI is conversion of 14 nucleotides of SAGE tag to a hundred bases of 3¢ cDNA fragment This figure is reproduced from [34] Fig Schematic of the high-throughput GLGI ... 38 lL of 2-propanol Precipitation mixture III: 64 lL of a 100% ethanol ⁄ m NaAc mixture (25 : 1), lL of glycogen (20 mgÆmL)1; Roche), and 10 lL of H2O Master sequencing mixtures: 0.7 lL of Big-Dye...
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THE CONSTITUTION OF LAW Legality in a Time of Emergency potx
... requires the participation of the legislature and the executive David Dyzenhaus is Professor of Law and Philosophy at the University of Toronto He is a Fellow of the Royal Society of Canada, and has ... Acknowledgements page ix Table of cases xi Table of statutes xiv Introduction 1 Legality in a time of emergency 17 Introduction 17 Judges and the politics of the rule of law Carl Schmitt’s challenge ... read most of the manuscript and gave me very helpful comments Finally, Luc Tremblay of the Faculty of Law of the University of Montreal arranged a seminar on the penultimate version of the manuscript...
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THE CONSTITUTION OF LAW Legality in a Time of Emergency docx
... requires the participation of the legislature and the executive David Dyzenhaus is Professor of Law and Philosophy at the University of Toronto He is a Fellow of the Royal Society of Canada, and has ... Acknowledgements page ix Table of cases xi Table of statutes xiv Introduction 1 Legality in a time of emergency 17 Introduction 17 Judges and the politics of the rule of law Carl Schmitt’s challenge ... read most of the manuscript and gave me very helpful comments Finally, Luc Tremblay of the Faculty of Law of the University of Montreal arranged a seminar on the penultimate version of the manuscript...
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Báo cáo khoa học: Effect of annealing time of an ice crystal on the activity of type III antifreeze protein pdf
... size of the ice crystal The lack of significant size dependence of TH activity (Fig 4B, open circles) might be ascribed to nonstabilization of the weak point of any size of the crystals, because of ... with the idea of progressive binding of AFP over a time scale of 2–3 h A larger number of convex ice fronts are presumably located on a larger-sized ice crystal If secondary binding of AFP has the ... annealing time and ice crystal size on TH (A) Thermal hysteresis of nfeAFP8 measured after annealing of an ice bipyramid for a certain period of time (0–3 h) The measurement was repeated for three times...
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Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot
... & Whitehouse CM (1989) Electrospray ionization for mass spectrometry of large biomolecules Science 246, 6471 52 Loo JA (2000) Electrospray ionization mass spectrometry: a technology for studying ... Int J Mass Spectrom 200, 175 186 53 Daniel JM, Friess SD, Rajagopalan S, Wendt S & Zenobi R (2002) Quantitative determination of noncovalent binding interactions using soft ionization mass spectrometry ... (1998) Fourier transform ion cyclotron resonance mass spectrometry: a primer Mass Spectrom Rev 17, 135 56 Smith RD (2000) Evolution of ESI -mass spectrometry and Fourier transform ion cyclotron...
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